English translation for "template rna"
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- 核糖核酸模板
信使核糖核酸
Related Translations:
- Example Sentences:
| 1. | After transcription , we gained the subgenomic rna that may play the role of the template rna recognized by den rdrp
经转录反应获得den亚基因组rna ，即为denrdrp可特异识别的模板rna 。 | | 2. | The ns5 protein of dengue virus is the largest molecule encoded by the virus genome with a molecule weight of 104 000 . recent research work indicates that the ns5 protein acts as the rna - dependent rna polymerase ( rdrp ) in virus which has the ability to recognize and bind its template rna to synthesize a complementary strand
近年来的研究表明， ns5蛋白具有rna依赖的rna聚合酶( rdrp )功能，即可以识别具有相对特异性的模板rna并与之结合，合成与模板互补的rna链，在病毒基因组复制过程中起关键作用。 | | 3. | In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
扩增产物连接到pgem - teasy载体中，转化大肠杆菌dh5菌株，筛选氨苄青霉素抗性菌落，提取质粒经酶切鉴定、 pcr分析以及确证性测序证明，所克隆的1500bp左右的片段含有完整的3abc基因，与国外参考序列相比，同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后，亚克隆3abc基因至原核表达载体ptriex - 4neo中，通过酶切鉴定、 pcr扩增以及序列分析，发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失，碰巧在3ab基因后形成一终止密码子，但3ab基因的阅读框架完整，选出含有3ab基因完整阅读框架的阳性克隆，用iptg诱导表达，收集菌液进行sds - page电泳、 westernblotting分析，结果表明， 3ab基因在大肠杆菌中成功表达，其表达产物为分子量33 . 5ku的融合蛋白，并能被口蹄疫病毒阳性血清识别。经薄层扫描分析，表达量占总蛋白量的26以上。 |
- Similar Words：
- "template polymorphism" English translation, "template positioning" English translation, "template process" English translation, "template reaction" English translation, "template ribonucleic acid" English translation, "template scan" English translation, "template set" English translation, "template shaping press" English translation, "template sho" English translation, "template shop" English translation
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